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KMID : 1094720220270050869
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Biotechnology and Bioprocess Engineering
2022 Volume.27 No. 5 p.869 ~ p.878
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Ultrasound-assisted Extraction of Polyphenols from Carthamus tinctorius Seeds: Optimization of Process Variables
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Kim Jun-Hee
Yeom Suh-Hee
Hwang Youn-Seon
Kim So-Hee
Kim Jin-Woo
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Abstract
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In this study, optimization of ultrasound-assisted extraction (UAE) conditions was conducted to increase the production of bioactive components from Carthamus tinctorius seeds (CS) using statistically-based optimization. The effects of major independent variables including extraction time (5.0?55.0 min), extraction temperature (26.0?94.0°C), and ethanol concentration (0.0?99.5%) were optimized using central composite design (CCD) to explore conditions that simultaneously maximize total polyphenol content, total flavonoid content, and radical scavenging activity. According to quadratic regression models, the predicted optimum UAE conditions were 33.7 min, 75.1°C, and 68.3% ethanol, respectively. Under these conditions, the experimental results were found to be 9.94 mg gallic acid equivalents/g dry matter (DM) of TPC, 0.28 mg quercetin equivalents/g DM of TFC, and 66.6% of RSA which are in good agreement with the predicted values. CS extract was subjected to LC/MS/MS analysis to analyze components with bioactive activities, and apigenin was determined as the main component. Therefore, we have concluded that UAE is an effective process for extracting antioxidant components and CS extract with bioactive components can be utilized as food, cosmetics, and pharmaceutical materials.
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KEYWORD
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Carthamus tinctorius seeds, central composite design, ultrasound-assisted extraction, antioxidant, apigenin
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